Composite

Part:BBa_I721005:Design

Designed by: Adam Emrich   Group: iGEM07_Brown   (2007-10-26)


pTet>ERBS>AraC>ERBS>LacI>ERBS>ECFP+AAV>Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed to repress the other 2 pathways involved in the Tristable Switch. Also designed to indicate when it is being expressed.

Source

Composite part formed from available basic parts.

References